Review



anti spon1  (Bio-Techne corporation)


Bioz Verified Symbol Bio-Techne corporation is a verified supplier
Bioz Manufacturer Symbol Bio-Techne corporation manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Bio-Techne corporation anti spon1
    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. <t>SPON1,</t> NTN1, and NRP2 are included as conserved controls between species.
    Anti Spon1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/Human+F-Spondin%2FSPON1+Antibody/bio_rxiv__2024__06__26__600229-262-35-39
    Average 90 stars, based on 4 article reviews
    anti spon1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Midline Assembloids Reveal Regulators of Human Axon Guidance"

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance

    Journal: bioRxiv

    doi: 10.1101/2024.06.26.600229

    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. SPON1, NTN1, and NRP2 are included as conserved controls between species.
    Figure Legend Snippet: (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. SPON1, NTN1, and NRP2 are included as conserved controls between species.

    Techniques Used: Membrane, Liquid Chromatography with Mass Spectroscopy, Expressing

    (A) Comparison of gene expression levels of 14,852 orthologous genes (gray points) between mouse (E9-14) and human (CS12-19) FP cells. Black dotted line indicates 1:1 gene expression while the blue dotted lines indicate 2-and 10-fold human enrichment. Colored points indicate membrane and secreted FP-enriched genes and indicate degree of gene expression divergence between human and mouse. (B) Schematic of workflow for generating hFpO from pooled KO hiPS cells and subsequently assembling with day 22 hSpO to form hMA in an arrayed format. At 3 d.a.f., the assembloids were cleared and confocal imaged to visualize commissural axons guiding towards the hFpO. (C) Heatmap displaying min/max normalized, fold-change in ROBO3 fluorescence intensity of hSpO axons 150 μm pre- and 150 μm post-crossing into the hFpO (n = 2-3 assembloids per gene). Two-way ANOVA performed on measurements the most distal 50 μm of heatmap; F 31,1972 = 10.05, P < 0.0001, following Dunnett’s multiple comparison test comparing Control 1 to all other conditions: ****P <0.0001 for Control 1 vs GALNT2 , ****P <0.0001 for Control 1 vs PLD3 , ****P < 0.0001 for Control 1 vs NTN1 , and **P = 0.0025 for Control 1 vs SPON1 . (D) Representative z-projected immunocytochemistry images of control and pooled KO hMA expressing ROBO3 and FOXA2 to visualize ROBO3+ axons projecting across the hFpO boundary. Scale bar, 100 μm. (E) Plot profiles of normalized fold-change ROBO3 fluorescence intensity of KO pool hFpO hMA 150 μm pre- and 150 μm post-crossing into the hFpO from (C). Dotted lines indicate the non-targeting control groups while the solid lines indicate the target gene group. Data is presented as mean ± SEM. Gray shaded region indicates the 50 μm bin where ROBO3 expression as a measure of axon guidance was assessed for statistical significance in (C). (F) Schematic outlining the method for generating isogenic KO hiPS cell lines from the axon guidance screen candidates and subsequent isogenic validation of phenotypes identified in the axon guidance screen. (G) Representative z-projected immunocytochemistry images of control and isogenic KO hMA expressing ROBO3 and FOXA2 at 2 d.a.f. Scale bar, 200 μm. (H) Bar plot displaying the proportion of ROBO3 coverage relative to the area of the isogenic KO hFpO candidates from 1-3 d.a.f (shades of grey; n = 4-5 assembloids per condition). Data is presented as mean ± SEM. Two-way ANOVA, F 4,59 = 121.8, P < 0.0001, following Tukey’s multiple comparison test ****P < 0.0001.
    Figure Legend Snippet: (A) Comparison of gene expression levels of 14,852 orthologous genes (gray points) between mouse (E9-14) and human (CS12-19) FP cells. Black dotted line indicates 1:1 gene expression while the blue dotted lines indicate 2-and 10-fold human enrichment. Colored points indicate membrane and secreted FP-enriched genes and indicate degree of gene expression divergence between human and mouse. (B) Schematic of workflow for generating hFpO from pooled KO hiPS cells and subsequently assembling with day 22 hSpO to form hMA in an arrayed format. At 3 d.a.f., the assembloids were cleared and confocal imaged to visualize commissural axons guiding towards the hFpO. (C) Heatmap displaying min/max normalized, fold-change in ROBO3 fluorescence intensity of hSpO axons 150 μm pre- and 150 μm post-crossing into the hFpO (n = 2-3 assembloids per gene). Two-way ANOVA performed on measurements the most distal 50 μm of heatmap; F 31,1972 = 10.05, P < 0.0001, following Dunnett’s multiple comparison test comparing Control 1 to all other conditions: ****P <0.0001 for Control 1 vs GALNT2 , ****P <0.0001 for Control 1 vs PLD3 , ****P < 0.0001 for Control 1 vs NTN1 , and **P = 0.0025 for Control 1 vs SPON1 . (D) Representative z-projected immunocytochemistry images of control and pooled KO hMA expressing ROBO3 and FOXA2 to visualize ROBO3+ axons projecting across the hFpO boundary. Scale bar, 100 μm. (E) Plot profiles of normalized fold-change ROBO3 fluorescence intensity of KO pool hFpO hMA 150 μm pre- and 150 μm post-crossing into the hFpO from (C). Dotted lines indicate the non-targeting control groups while the solid lines indicate the target gene group. Data is presented as mean ± SEM. Gray shaded region indicates the 50 μm bin where ROBO3 expression as a measure of axon guidance was assessed for statistical significance in (C). (F) Schematic outlining the method for generating isogenic KO hiPS cell lines from the axon guidance screen candidates and subsequent isogenic validation of phenotypes identified in the axon guidance screen. (G) Representative z-projected immunocytochemistry images of control and isogenic KO hMA expressing ROBO3 and FOXA2 at 2 d.a.f. Scale bar, 200 μm. (H) Bar plot displaying the proportion of ROBO3 coverage relative to the area of the isogenic KO hFpO candidates from 1-3 d.a.f (shades of grey; n = 4-5 assembloids per condition). Data is presented as mean ± SEM. Two-way ANOVA, F 4,59 = 121.8, P < 0.0001, following Tukey’s multiple comparison test ****P < 0.0001.

    Techniques Used: Comparison, Expressing, Membrane, Fluorescence, Control, Immunocytochemistry

    (A) Inference of CRISPR edits (ICE; no border) and KO-score (border) of CRISPR edited hFpO at day 8 (dark blue) and day 11 (light blue) of differentiation. (B) Gene expression analysis (by RT-qPCR) of FOXA2 in control and CRISPR edited hFpO at day 8 and 11 of differentiation (pooled mRNA from 3 individual organoids). Color scale indicates the relative expression of FOXA2 normalized to GAPDH . (C) Representative brightfield images of control and CRISPR edited hiPS cells (top) and z-projection of immunocytochemical images hFpO (stained for FOXA2 and Reddot2 nuclear stain) at day 8 and 11 of differentiation (bottom). Scale bar 100 μm. (D) Quantification of FOXA2/RedDot2 fluorescence intensity of control and CRISPR edited hFpO at day 8 (light gray) and 11 (dark gray) of differentiation (n = 2-3 organoids per condition). Data is normalized to negative control (hSpO). Data is presented as mean ± SEM. (E) ICE (no border) and KO-score (border) of isogenic CRISPR edited hiPS cell lines for GALNT2, NTN1 , and SPON1 knockout. (F) Western blot validation of hSpO and hFpO cell extracts and conditioned media collected from control and isogenic KO for GALNT2 (top), NTN1 (middle), and SPON1 (bottom). GAPDH immunoblotting was used as the sample loading control. (G) Gene expression analysis (by RT-qPCR) of FOXA2, PAX6 , and NKX2 . 2 in control and isogenic CRISPR edited hFpO at day 8 of differentiation. Each dot represents a separate differentiation experiment normalized to GAPDH expression. Data is presented as mean ± SEM. Two-tailed Mann-Whitney test was used. For FOXA2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For NKX2-2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For PAX6 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; P = 0.0571.
    Figure Legend Snippet: (A) Inference of CRISPR edits (ICE; no border) and KO-score (border) of CRISPR edited hFpO at day 8 (dark blue) and day 11 (light blue) of differentiation. (B) Gene expression analysis (by RT-qPCR) of FOXA2 in control and CRISPR edited hFpO at day 8 and 11 of differentiation (pooled mRNA from 3 individual organoids). Color scale indicates the relative expression of FOXA2 normalized to GAPDH . (C) Representative brightfield images of control and CRISPR edited hiPS cells (top) and z-projection of immunocytochemical images hFpO (stained for FOXA2 and Reddot2 nuclear stain) at day 8 and 11 of differentiation (bottom). Scale bar 100 μm. (D) Quantification of FOXA2/RedDot2 fluorescence intensity of control and CRISPR edited hFpO at day 8 (light gray) and 11 (dark gray) of differentiation (n = 2-3 organoids per condition). Data is normalized to negative control (hSpO). Data is presented as mean ± SEM. (E) ICE (no border) and KO-score (border) of isogenic CRISPR edited hiPS cell lines for GALNT2, NTN1 , and SPON1 knockout. (F) Western blot validation of hSpO and hFpO cell extracts and conditioned media collected from control and isogenic KO for GALNT2 (top), NTN1 (middle), and SPON1 (bottom). GAPDH immunoblotting was used as the sample loading control. (G) Gene expression analysis (by RT-qPCR) of FOXA2, PAX6 , and NKX2 . 2 in control and isogenic CRISPR edited hFpO at day 8 of differentiation. Each dot represents a separate differentiation experiment normalized to GAPDH expression. Data is presented as mean ± SEM. Two-tailed Mann-Whitney test was used. For FOXA2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For NKX2-2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For PAX6 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; P = 0.0571.

    Techniques Used: CRISPR, Expressing, Quantitative RT-PCR, Control, Staining, Fluorescence, Negative Control, Knock-Out, Western Blot, Two Tailed Test, MANN-WHITNEY

    Related Articles

    Membrane:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Expressing:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Comparison:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Fluorescence:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Control:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Immunocytochemistry:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    CRISPR:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Quantitative RT-PCR:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Staining:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Negative Control:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Knock-Out:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Western Blot:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Two Tailed Test:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    MANN-WHITNEY:

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance
    Article Snippet: expression was visualized with chemiluminescent substrate (PerkinElmer, NEL113001EA). .. The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).



    Similar Products

    anti spon1  (Bio-Techne corporation)
    90
    Bio-Techne corporation anti spon1
    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. <t>SPON1,</t> NTN1, and NRP2 are included as conserved controls between species.
    Anti Spon1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/Human+F-Spondin%2FSPON1+Antibody/bio_rxiv__2024__06__26__600229-262-35-39
    Average 90 stars, based on 1 article reviews
    anti spon1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    anti spon1  (Bioss)
    92
    Bioss anti spon1
    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. <t>SPON1,</t> NTN1, and NRP2 are included as conserved controls between species.
    Anti Spon1, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/SPON1+Polyclonal+Antibody/pm36520032-110-13-19
    Average 92 stars, based on 1 article reviews
    anti spon1 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    anti spon1 polyclonal antibody  (R&D Systems)
    90
    R&D Systems anti spon1 polyclonal antibody
    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. <t>SPON1,</t> NTN1, and NRP2 are included as conserved controls between species.
    Anti Spon1 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/Human+F-Spondin%2FSPON1+Antibody/pm41947214-100-3-9
    Average 90 stars, based on 1 article reviews
    anti spon1 polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti- spon1 antibody  (Thermo Fisher)
    90
    Thermo Fisher rabbit polyclonal anti- spon1 antibody
    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. <t>SPON1,</t> NTN1, and NRP2 are included as conserved controls between species.
    Rabbit Polyclonal Anti Spon1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/pooled+taqman+gene+expression+assay+probes+cck/10__1161_slash_jaha__123__032888-73-6-15
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti- spon1 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    spon1  (Signalway Antibody)
    86
    Signalway Antibody spon1
    Immunohistochemical staining of <t>Spon1</t> of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).
    Spon1, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/anti+spon1/pmc12651710-114-27-30
    Average 86 stars, based on 1 article reviews
    spon1 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti–spon1 (spondin 1) antibody #pa5–116176  (Thermo Fisher)
    90
    Thermo Fisher rabbit polyclonal anti–spon1 (spondin 1) antibody #pa5–116176
    Immunohistochemical staining of <t>Spon1</t> of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).
    Rabbit Polyclonal Anti–Spon1 (Spondin 1) Antibody #Pa5–116176, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/pooled+taqman+gene+expression+assay+probes+cck/pmc11255731-119-8-13
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti–spon1 (spondin 1) antibody #pa5–116176 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    anti-spon1  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology anti-spon1
    Immunohistochemical staining of <t>Spon1</t> of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).
    Anti Spon1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/anti+spon1/10__3390_slash_neuroglia4010006-48-62-69
    Average 90 stars, based on 1 article reviews
    anti-spon1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    spon1  (OriGene)
    91
    OriGene spon1
    Immunohistochemical staining of <t>Spon1</t> of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).
    Spon1, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/SPON1+Rabbit+Polyclonal+Antibody/pmc09892830-138-30-36
    Average 91 stars, based on 1 article reviews
    spon1 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    anti spon1 polyclonal antibody  (ProSci Incorporated)
    90
    ProSci Incorporated anti spon1 polyclonal antibody
    Immunohistochemical staining of <t>Spon1</t> of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).
    Anti Spon1 Polyclonal Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+spon1/SPON1+Antibody/pm22499432-82-11-16
    Average 90 stars, based on 1 article reviews
    anti spon1 polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. SPON1, NTN1, and NRP2 are included as conserved controls between species.

    Journal: bioRxiv

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance

    doi: 10.1101/2024.06.26.600229

    Figure Lengend Snippet: (A) Venn diagram outlining filtering for membrane and secreted genes that are enriched in the FP. The list was curated based on LC-MS identification of proteins from hFpO conditioned media, enriched expression in primary human FP cells from scRNA seq datasets, and presence in membrane/secreted GO terms. (B) Graph displaying ranked fold-difference in expression of membrane/secreted FP genes ordered from most conserved to most divergent expression. Heatmap represents the proportion of studies that found the plotted genes to be essential from the DepMap Portal. (C) Violin plots of gene expression for the most (>10-fold) divergently expressed membrane/secreted in humans (left) compared to mouse (right) primary FP cells. SPON1, NTN1, and NRP2 are included as conserved controls between species.

    Article Snippet: The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Techniques: Membrane, Liquid Chromatography with Mass Spectroscopy, Expressing

    (A) Comparison of gene expression levels of 14,852 orthologous genes (gray points) between mouse (E9-14) and human (CS12-19) FP cells. Black dotted line indicates 1:1 gene expression while the blue dotted lines indicate 2-and 10-fold human enrichment. Colored points indicate membrane and secreted FP-enriched genes and indicate degree of gene expression divergence between human and mouse. (B) Schematic of workflow for generating hFpO from pooled KO hiPS cells and subsequently assembling with day 22 hSpO to form hMA in an arrayed format. At 3 d.a.f., the assembloids were cleared and confocal imaged to visualize commissural axons guiding towards the hFpO. (C) Heatmap displaying min/max normalized, fold-change in ROBO3 fluorescence intensity of hSpO axons 150 μm pre- and 150 μm post-crossing into the hFpO (n = 2-3 assembloids per gene). Two-way ANOVA performed on measurements the most distal 50 μm of heatmap; F 31,1972 = 10.05, P < 0.0001, following Dunnett’s multiple comparison test comparing Control 1 to all other conditions: ****P <0.0001 for Control 1 vs GALNT2 , ****P <0.0001 for Control 1 vs PLD3 , ****P < 0.0001 for Control 1 vs NTN1 , and **P = 0.0025 for Control 1 vs SPON1 . (D) Representative z-projected immunocytochemistry images of control and pooled KO hMA expressing ROBO3 and FOXA2 to visualize ROBO3+ axons projecting across the hFpO boundary. Scale bar, 100 μm. (E) Plot profiles of normalized fold-change ROBO3 fluorescence intensity of KO pool hFpO hMA 150 μm pre- and 150 μm post-crossing into the hFpO from (C). Dotted lines indicate the non-targeting control groups while the solid lines indicate the target gene group. Data is presented as mean ± SEM. Gray shaded region indicates the 50 μm bin where ROBO3 expression as a measure of axon guidance was assessed for statistical significance in (C). (F) Schematic outlining the method for generating isogenic KO hiPS cell lines from the axon guidance screen candidates and subsequent isogenic validation of phenotypes identified in the axon guidance screen. (G) Representative z-projected immunocytochemistry images of control and isogenic KO hMA expressing ROBO3 and FOXA2 at 2 d.a.f. Scale bar, 200 μm. (H) Bar plot displaying the proportion of ROBO3 coverage relative to the area of the isogenic KO hFpO candidates from 1-3 d.a.f (shades of grey; n = 4-5 assembloids per condition). Data is presented as mean ± SEM. Two-way ANOVA, F 4,59 = 121.8, P < 0.0001, following Tukey’s multiple comparison test ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance

    doi: 10.1101/2024.06.26.600229

    Figure Lengend Snippet: (A) Comparison of gene expression levels of 14,852 orthologous genes (gray points) between mouse (E9-14) and human (CS12-19) FP cells. Black dotted line indicates 1:1 gene expression while the blue dotted lines indicate 2-and 10-fold human enrichment. Colored points indicate membrane and secreted FP-enriched genes and indicate degree of gene expression divergence between human and mouse. (B) Schematic of workflow for generating hFpO from pooled KO hiPS cells and subsequently assembling with day 22 hSpO to form hMA in an arrayed format. At 3 d.a.f., the assembloids were cleared and confocal imaged to visualize commissural axons guiding towards the hFpO. (C) Heatmap displaying min/max normalized, fold-change in ROBO3 fluorescence intensity of hSpO axons 150 μm pre- and 150 μm post-crossing into the hFpO (n = 2-3 assembloids per gene). Two-way ANOVA performed on measurements the most distal 50 μm of heatmap; F 31,1972 = 10.05, P < 0.0001, following Dunnett’s multiple comparison test comparing Control 1 to all other conditions: ****P <0.0001 for Control 1 vs GALNT2 , ****P <0.0001 for Control 1 vs PLD3 , ****P < 0.0001 for Control 1 vs NTN1 , and **P = 0.0025 for Control 1 vs SPON1 . (D) Representative z-projected immunocytochemistry images of control and pooled KO hMA expressing ROBO3 and FOXA2 to visualize ROBO3+ axons projecting across the hFpO boundary. Scale bar, 100 μm. (E) Plot profiles of normalized fold-change ROBO3 fluorescence intensity of KO pool hFpO hMA 150 μm pre- and 150 μm post-crossing into the hFpO from (C). Dotted lines indicate the non-targeting control groups while the solid lines indicate the target gene group. Data is presented as mean ± SEM. Gray shaded region indicates the 50 μm bin where ROBO3 expression as a measure of axon guidance was assessed for statistical significance in (C). (F) Schematic outlining the method for generating isogenic KO hiPS cell lines from the axon guidance screen candidates and subsequent isogenic validation of phenotypes identified in the axon guidance screen. (G) Representative z-projected immunocytochemistry images of control and isogenic KO hMA expressing ROBO3 and FOXA2 at 2 d.a.f. Scale bar, 200 μm. (H) Bar plot displaying the proportion of ROBO3 coverage relative to the area of the isogenic KO hFpO candidates from 1-3 d.a.f (shades of grey; n = 4-5 assembloids per condition). Data is presented as mean ± SEM. Two-way ANOVA, F 4,59 = 121.8, P < 0.0001, following Tukey’s multiple comparison test ****P < 0.0001.

    Article Snippet: The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Techniques: Comparison, Expressing, Membrane, Fluorescence, Control, Immunocytochemistry

    (A) Inference of CRISPR edits (ICE; no border) and KO-score (border) of CRISPR edited hFpO at day 8 (dark blue) and day 11 (light blue) of differentiation. (B) Gene expression analysis (by RT-qPCR) of FOXA2 in control and CRISPR edited hFpO at day 8 and 11 of differentiation (pooled mRNA from 3 individual organoids). Color scale indicates the relative expression of FOXA2 normalized to GAPDH . (C) Representative brightfield images of control and CRISPR edited hiPS cells (top) and z-projection of immunocytochemical images hFpO (stained for FOXA2 and Reddot2 nuclear stain) at day 8 and 11 of differentiation (bottom). Scale bar 100 μm. (D) Quantification of FOXA2/RedDot2 fluorescence intensity of control and CRISPR edited hFpO at day 8 (light gray) and 11 (dark gray) of differentiation (n = 2-3 organoids per condition). Data is normalized to negative control (hSpO). Data is presented as mean ± SEM. (E) ICE (no border) and KO-score (border) of isogenic CRISPR edited hiPS cell lines for GALNT2, NTN1 , and SPON1 knockout. (F) Western blot validation of hSpO and hFpO cell extracts and conditioned media collected from control and isogenic KO for GALNT2 (top), NTN1 (middle), and SPON1 (bottom). GAPDH immunoblotting was used as the sample loading control. (G) Gene expression analysis (by RT-qPCR) of FOXA2, PAX6 , and NKX2 . 2 in control and isogenic CRISPR edited hFpO at day 8 of differentiation. Each dot represents a separate differentiation experiment normalized to GAPDH expression. Data is presented as mean ± SEM. Two-tailed Mann-Whitney test was used. For FOXA2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For NKX2-2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For PAX6 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; P = 0.0571.

    Journal: bioRxiv

    Article Title: Midline Assembloids Reveal Regulators of Human Axon Guidance

    doi: 10.1101/2024.06.26.600229

    Figure Lengend Snippet: (A) Inference of CRISPR edits (ICE; no border) and KO-score (border) of CRISPR edited hFpO at day 8 (dark blue) and day 11 (light blue) of differentiation. (B) Gene expression analysis (by RT-qPCR) of FOXA2 in control and CRISPR edited hFpO at day 8 and 11 of differentiation (pooled mRNA from 3 individual organoids). Color scale indicates the relative expression of FOXA2 normalized to GAPDH . (C) Representative brightfield images of control and CRISPR edited hiPS cells (top) and z-projection of immunocytochemical images hFpO (stained for FOXA2 and Reddot2 nuclear stain) at day 8 and 11 of differentiation (bottom). Scale bar 100 μm. (D) Quantification of FOXA2/RedDot2 fluorescence intensity of control and CRISPR edited hFpO at day 8 (light gray) and 11 (dark gray) of differentiation (n = 2-3 organoids per condition). Data is normalized to negative control (hSpO). Data is presented as mean ± SEM. (E) ICE (no border) and KO-score (border) of isogenic CRISPR edited hiPS cell lines for GALNT2, NTN1 , and SPON1 knockout. (F) Western blot validation of hSpO and hFpO cell extracts and conditioned media collected from control and isogenic KO for GALNT2 (top), NTN1 (middle), and SPON1 (bottom). GAPDH immunoblotting was used as the sample loading control. (G) Gene expression analysis (by RT-qPCR) of FOXA2, PAX6 , and NKX2 . 2 in control and isogenic CRISPR edited hFpO at day 8 of differentiation. Each dot represents a separate differentiation experiment normalized to GAPDH expression. Data is presented as mean ± SEM. Two-tailed Mann-Whitney test was used. For FOXA2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For NKX2-2 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; *P = 0.0286. For PAX6 : n = 4 control and 4 GALNT2 KO from 1 hiPS cell line; P = 0.0571.

    Article Snippet: The following primary antibodies were used: anti-NTN1 antibody (1:1000 dilution, rabbit, Abcam, ab126729), anti-SHH antibody (1:1000 dilution, rabbit, Abcam, ab53281), anti-GAPDH (1:3000 dilution, mouse, Cell Signaling Technology, 2118S), anti-GALNT2 (1:1000 dilution, rabbit, Invitrogen, PA5-21541), and anti-SPON1 (1:1000 dilution, goat, Bio-Techne, AF3135).

    Techniques: CRISPR, Expressing, Quantitative RT-PCR, Control, Staining, Fluorescence, Negative Control, Knock-Out, Western Blot, Two Tailed Test, MANN-WHITNEY

    Immunohistochemical staining of Spon1 of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).

    Journal: Dentistry Journal

    Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

    doi: 10.3390/dj13110494

    Figure Lengend Snippet: Immunohistochemical staining of Spon1 of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).

    Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

    Techniques: Immunohistochemical staining, Staining, Control

    mRNA expression levels at each BMP-7 concentration assessed by qRT-PCR analysis. The expression levels of each mRNA were higher in the 100 ng/mL BMP-7-treated group compared to the control group. ( A ) SPON1 mRNA expression levels. ( B ) CEMP1 mRNA expression levels. ( C ) RUNX2 mRNA expression levels. ( D ) BGLAP mRNA expression levels. ( n = 4) Asterisks indicate significant differences among BMP-7 concentrations on the same day (*: p < 0.05, **: p < 0.01), whereas daggers indicate significant differences between Day 7 and Day 14 within the same BMP-7 concentration (†: p < 0.05, ††: p < 0.01).

    Journal: Dentistry Journal

    Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

    doi: 10.3390/dj13110494

    Figure Lengend Snippet: mRNA expression levels at each BMP-7 concentration assessed by qRT-PCR analysis. The expression levels of each mRNA were higher in the 100 ng/mL BMP-7-treated group compared to the control group. ( A ) SPON1 mRNA expression levels. ( B ) CEMP1 mRNA expression levels. ( C ) RUNX2 mRNA expression levels. ( D ) BGLAP mRNA expression levels. ( n = 4) Asterisks indicate significant differences among BMP-7 concentrations on the same day (*: p < 0.05, **: p < 0.01), whereas daggers indicate significant differences between Day 7 and Day 14 within the same BMP-7 concentration (†: p < 0.05, ††: p < 0.01).

    Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

    Techniques: Expressing, Concentration Assay, Quantitative RT-PCR, Control

    Detection of proteins in HPLFs following treatment at each BMP-7 concentration by Western blot analysis. SPON1 and CEMP1 proteins were qualitatively detected, showing the strongest bands in the 100 ng/mL BMP-7-treated group, followed by the 200 ng/mL BMP-7-treated group, whereas minimal or no expression was observed in the control. Lane 1, 0 ng/mL BMP-7-treated group as a control; lane 2, 100 ng/mL BMP-7-treated group; lane 3, 200 ng/mL BMP-7-treated group. Representative qualitative results are shown.

    Journal: Dentistry Journal

    Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

    doi: 10.3390/dj13110494

    Figure Lengend Snippet: Detection of proteins in HPLFs following treatment at each BMP-7 concentration by Western blot analysis. SPON1 and CEMP1 proteins were qualitatively detected, showing the strongest bands in the 100 ng/mL BMP-7-treated group, followed by the 200 ng/mL BMP-7-treated group, whereas minimal or no expression was observed in the control. Lane 1, 0 ng/mL BMP-7-treated group as a control; lane 2, 100 ng/mL BMP-7-treated group; lane 3, 200 ng/mL BMP-7-treated group. Representative qualitative results are shown.

    Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

    Techniques: Concentration Assay, Western Blot, Expressing, Control